Immobilisation of enzymes on super paramagnetic nanoparticles for biodiesel production
Başlık çevirisi mevcut değil.
- Tez No: 403114
- Danışmanlar: Belirtilmemiş.
- Tez Türü: Yüksek Lisans
- Konular: Metalurji Mühendisliği, Metallurgical Engineering
- Anahtar Kelimeler: Belirtilmemiş.
- Yıl: 2015
- Dil: İngilizce
- Üniversite: Unıversıty Of Central Lancashıre (uclan)
- Enstitü: Yurtdışı Enstitü
- Ana Bilim Dalı: Belirtilmemiş.
- Bilim Dalı: Belirtilmemiş.
- Sayfa Sayısı: Belirtilmemiş.
Özet
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Özet (Çeviri)
Commercial material DAE was modified by the incorporation iron oxide by one pot synthesis, was characterised by several techniques i.e. Energy Dispersive X-ray Analysis (EDAX), Powder X-ray Diffraction (XRD, Scanning Electron Microscope (SEM),) and (Transmission Electron Microscope (TEM). EDAX/SEM analyses confirmed the presence of iron oxide in the nanocomposites, (XRD) measurements showed the crystalline formation of the iron oxide nanocomposite. TEM and SEM with have been used for the determination of sizes, internal structure, and morphology of the composite. Furthermore, TEM images showed the porous structure of the DAE. While, FTIR spectroscopy was used for the determination for the presence to Fe–O bonding due to a specific stretching vibration at 551cm-1. On one hand, second part of the dissertation was consists of the surface functionalisation of the developed composed material. The composite was functionalised by silanisation (a classical method) with two different amino-silane groups i.e. APTS and APDS and was followed by the conversion of surface amine group into the aldehyde by using glutaraldehyde through chemical conjugation method, The surface functionalization with APTS showed more amine density as compared to APDS due to more number of ethoxy group attached with APTS. The functionalised nanocomposite was prepared on a small scale and was further altered by chemical conjugation with enzymes. The final part of this project was based on the biolytic applications of the developed and surface functionalised material. In this studies Candida Rugosa lipase (CRL)] were chemically conjugated through glutaraldehyde-modification onto the amino-functionalised nanoparticles for applications such as the formation of p-nitrophenol and palmitic acid, by the hydrolysis of p-nitrophenyl palmitate. It was observed from the bio catalytic reaction (i) that the conversion values given by lipase-immobilised materials were comparable to those given by free lipases with the added advantage of being reusable for further catalytic cycles.
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