Üreter tıkanıklıklığının sıçan böbrek dokusunda oluşturduğu histolojik değişiklikler
Histological alterations in rat kidney following ureteral obsruction
- Tez No: 59262
- Danışmanlar: PROF. DR. TÜLİN BAYKAL
- Tez Türü: Doktora
- Konular: Morfoloji, Morphology
- Anahtar Kelimeler: Böbrek, Üreter tıkanmaları, Kidney, Ureteral obstruction
- Yıl: 1997
- Dil: Türkçe
- Üniversite: Cumhuriyet Üniversitesi
- Enstitü: Sağlık Bilimleri Enstitüsü
- Ana Bilim Dalı: Histoloji ve Embriyoloji Ana Bilim Dalı
- Bilim Dalı: Belirtilmemiş.
- Sayfa Sayısı: Belirtilmemiş.
Özet
91 SUMMARY Histological alterations in rat kidney following ureteral obstruction In the present study, the effects of unilateral ureteral ligation on kidney morphology and function were determined both in the ligated side and in the contralateral side. 35 adult Swiss albino rats were used in this study. Animals were divided into 4 groups; 5 unoperated rats were used as the control group whereas the rest 30 were undergone the experimental procedure. Following the ligation of one ureter, animals were kept in standard laboratory conditions. 15, 30 and 45 days after operation, ten rats per group were killed and both kidneys from each animal were taken. Renal tissue was processed for both light and electron microscopy in order to determine the renal structural alterations following ureter obstruction. At the light microscope level while glomeruli of the kidneys from the ligated side showed degenerative changes which were increased with time, there were dilatations in distal and proximal tubuli on day 15, and these changes were followed by atrophic alterations on days 30 and 45. Interstitial tissue enlargement and leukocyte infiltration were the other findings. At the electron microscope level, ondulation and thickening of the glomerular basement membrane, irregularities and defects in the endothelial fenestrae and podocytes, and a decreased volume of urinary space were observed in the glomeruli. Although a decrease was seen in the number of intracytoplasmic foldings and mitochondria of the tubuli, a decrease was also evident in the number of lipid droplets and lysosomes. The basement membrane thickness of the proximal tubuli seemed to be increased. On day 45 of obstruction, most of the tubuli were atrophic in appearance. In the contralateral kidneys (unligated side), an increase in renal corpuscle size, also an increase in proximal tubular cell length and microvilli were observed. Distal tubular cells seemed to be flattened at the light microscopical level. At the electron92 microscopical levels, an increased glomerular basement membrane thickness Was seen whereas irregular endothelial cell cytoplasm and degenerative changes in podocytes were demonstrated in the other parts of the renal corpuscle. Although proximal tubular cell features were similar to those seen in the cpntrol group, dilatations in the number of vacuoles and lysosomes in the cytoplasm were evident. In addition, distal tubular cells showed cytoplasmic loss and abundant lysosomes in the contralateral side kidneys. The present study has shown that the long-term ureter obstruction caused irreversible structural and functional effects in the renal tissue. Uhligated side kidneys were also affected structurally by the obstruction process. In conclusion, it could be suggested that structural and functional defects of obstructed kidney, which plays a crucial role for homeostasis, may also have adverse effects on the contralateral side kidney. Therefore several problems may occur in the other parts of the body related to the homeostatic mechanisms.
Özet (Çeviri)
91 SUMMARY Histological alterations in rat kidney following ureteral obstruction In the present study, the effects of unilateral ureteral ligation on kidney morphology and function were determined both in the ligated side and in the contralateral side. 35 adult Swiss albino rats were used in this study. Animals were divided into 4 groups; 5 unoperated rats were used as the control group whereas the rest 30 were undergone the experimental procedure. Following the ligation of one ureter, animals were kept in standard laboratory conditions. 15, 30 and 45 days after operation, ten rats per group were killed and both kidneys from each animal were taken. Renal tissue was processed for both light and electron microscopy in order to determine the renal structural alterations following ureter obstruction. At the light microscope level while glomeruli of the kidneys from the ligated side showed degenerative changes which were increased with time, there were dilatations in distal and proximal tubuli on day 15, and these changes were followed by atrophic alterations on days 30 and 45. Interstitial tissue enlargement and leukocyte infiltration were the other findings. At the electron microscope level, ondulation and thickening of the glomerular basement membrane, irregularities and defects in the endothelial fenestrae and podocytes, and a decreased volume of urinary space were observed in the glomeruli. Although a decrease was seen in the number of intracytoplasmic foldings and mitochondria of the tubuli, a decrease was also evident in the number of lipid droplets and lysosomes. The basement membrane thickness of the proximal tubuli seemed to be increased. On day 45 of obstruction, most of the tubuli were atrophic in appearance. In the contralateral kidneys (unligated side), an increase in renal corpuscle size, also an increase in proximal tubular cell length and microvilli were observed. Distal tubular cells seemed to be flattened at the light microscopical level. At the electron92 microscopical levels, an increased glomerular basement membrane thickness Was seen whereas irregular endothelial cell cytoplasm and degenerative changes in podocytes were demonstrated in the other parts of the renal corpuscle. Although proximal tubular cell features were similar to those seen in the cpntrol group, dilatations in the number of vacuoles and lysosomes in the cytoplasm were evident. In addition, distal tubular cells showed cytoplasmic loss and abundant lysosomes in the contralateral side kidneys. The present study has shown that the long-term ureter obstruction caused irreversible structural and functional effects in the renal tissue. Uhligated side kidneys were also affected structurally by the obstruction process. In conclusion, it could be suggested that structural and functional defects of obstructed kidney, which plays a crucial role for homeostasis, may also have adverse effects on the contralateral side kidney. Therefore several problems may occur in the other parts of the body related to the homeostatic mechanisms.
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